Pro-Ovum



FP7-PEOPLE-2011-CIG, Research Executive Agency (REA)

Contract n. 303640 (Jan 2012- Jan 2016)


Principal Investigator: Valentina Lodde

Scientific Coordinator: Alberto M. Luciano

Project's permanent factsheet at:

http://cordis.europa.eu/project/rcn/102300

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  1. BulletProject: Role of Progesterone Receptor Membrane Component-1 in oogenesis and mammalian fertility (PRO-OVUM)


Summary


Infertility is a major problem that affects domestic mammals and declining fertility represents an obstacle in maintaining profitability of dairy farms in Europe and worldwide. Since declining fertility of cattle is mainly due to the poor quality of the oocytes, defining the factors and mechanisms that affect oocyte quality, is essential for improving female fertility. In particular, basic knowledge of which proteins within the oocyte regulate meiosis, oocyte fertilizability and early embryonic development would be advantageous. Based on our preliminary data, we propose that Progesterone Receptor Membrane Component 1 (PGRMC1) is one of the key factors that regulate mammalian oocyte quality. This hypothesis will be rigorously tested by determining 1) whether PGRMC1 plays an essential role in maintaining the ability of bovine oocytes to complete meiosis, undergo fertilization and develop to an healthy embryo in vitro, by genetically manipulating the levels of PGRMC1 within the oocyte, and 2) the PGRMC1 mechanism of action by identifying its interacting proteins within the oocyte.


Relevant pubblications 2012-2016


  1. 1.Franciosi F*, Lodde V*, Goudet G, Duchamp G, Deleuze S, Douet C, Tessaro I and Luciano AM. Changes in histone H4 acetylation during in vivo versus in vitro maturation of equine oocytes. Mol Hum Reprod 2012; 18:243-252.

  2. 2.Luciano AM, Lodde V, Franciosi F, Tessaro I, Corbani D and Modina S. Large-scale chromatin morpho-functional changes during mammalian oocyte growth and differentiation. Eur J Histochem 2012; 56:e37.

  3. 3.Peluso JJ, DeCerbo J and Lodde V. Evidence for a genomic mechanism of action for progesterone receptor membrane component-1. Steroids 2012; 77:1007-1012.

  4. 4.Peluso JJ, Lodde V and Liu X. Progesterone regulation of progesterone receptor membrane component 1 (PGRMC1) sumoylation and transcriptional activity in spontaneously immortalized granulosa cells. Endocrinology 2012; 153:3929-3939.

  5. 5.Dieci C*, Lodde V*, Franciosi F, Lagutina I, Tessaro I, Modina SC, Albertini DF, Lazzari G, Galli C and Luciano AM. The effect of cilostamide on gap junction communication dynamics, chromatin remodeling, and competence acquisition in pig oocytes following parthenogenetic activation and nuclear transfer. Biol Reprod 2013; 89:68.

  6. 6.Lodde V, Franciosi F, Tessaro I, Modina SC and Luciano AM. Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte. J Assist Reprod Genet 2013; 30:1219-1226.

  7. 7.Luciano AM, Franciosi F, Lodde V, Tessaro I, Corbani D, Modina SC and Peluso JJ. Oocytes isolated from dairy cows with reduced ovarian reserve have a high frequency of aneuploidy and alterations in the localization of progesterone receptor membrane component 1 and aurora kinase B. Biol Reprod 2013; 88:58.

  8. 8.Peluso JJ, Yuan A, Liu X and Lodde V. Plasminogen activator inhibitor 1 RNA-binding protein interacts with progesterone receptor membrane component 1 to regulate progesterone's ability to maintain the viability of spontaneously immortalized granulosa cells and rat granulosa cells. Biol Reprod 2013; 88:20.

  9. 9.Franciosi F, Coticchio G, Lodde V, Tessaro I, Modina SC, Fadini R, Dal Canto M, Renzini MM, Albertini DF and Luciano AM. Natriuretic peptide precursor C delays meiotic resumption and sustains gap junction-mediated communication in bovine cumulus-enclosed oocytes. Biol Reprod 2014; 91:61.

  10. 10.Luciano AM, Franciosi F, Dieci C and Lodde V. Changes in large-scale chromatin structure and function during oogenesis: a journey in company with follicular cells. Anim Reprod Sci 2014; 149:3-10.

  11. 11.Modina SC, Tessaro I, Lodde V, Franciosi F, Corbani D and Luciano AM. Reductions in the number of mid-sized antral follicles are associated with markers of premature ovarian senescence in dairy cows. Reprod Fertil Dev 2014; 26:235-244.

  12. 12.Franciosi F, Goudet G, Tessaro I, Papillier P, Dalbies-Tran R, Reigner F, Deleuze S, Douet C, Miclea I, Lodde V, Luciano AM. In vitro maturation affects chromosome segregation, spindle morphology and acetylation of lysine 16 on histone H4 in horse oocytes. Reprod Fertil Dev 2015:doi: 10.1071/RD15350.

  13. 13.Labrecque R, Lodde V, Dieci C, Tessaro I, Luciano AM, Sirard MA. Chromatin remodelling and histone mRNA accumulation in bovine germinal vesicle oocytes. Mol Reprod Dev 2015; 82:450-462.

  14. 14.Tessaro I, Modina SC, Crotti G, Franciosi F, Colleoni S, Lodde V, Galli C, Lazzari G, Luciano AM. Transferability and inter-laboratory variability assessment of the in vitro bovine oocyte fertilization test. Reprod Toxicol 2015; 51:106-113.

  15. 15.Tessaro I, Modina SC, Franciosi F, Sivelli G, Terzaghi L, Lodde V, Luciano AM. Effect of oral administration of low-dose follicle stimulating hormone on hyperandrogenized mice as a model of polycystic ovary syndrome. J Ovarian Res 2015; 8:64.

  16. 16.Terzaghi L, Tessaro I, Raucci F, Merico V, Mazzini G, Garagna S, Zuccotti M, Franciosi F, Lodde V. PGRMC1 participates in late events of bovine granulosa cells mitosis and oocyte meiosis. Cell Cycle 2016; 15:2019-2032.

  17. 17.Dieci C*, Lodde V*, Labreque R, Dufort I, Tessaro I, Sirard MA, Luciano AM. Differences in cumulus cell gene expression indicate the benefit of a pre-maturation step to improve in-vitro bovine embryo production. Mol Hum Reprod 2016; 10.1093/molehr/gaw055.